The strand specificity of the sequencing showed evidence for both sense and antisense transcripts at the 6S2 RNA locus, an observation subsequently confirmed using Northern Blots (Fig. 2).
Understanding the mechanisms that led to the diversification of this family would help decipher the specificity of the sequence-structure-function relationships of each sub-family and would improve drug design targeted to GPCRs.
For primers used in PCR and sequencing reactions see Table 1: All forward and reverse sequences were aligned for each amplicon using the ClustalW program to assess the specificity of the sequencing reactions (http://www.ebi.ac.uk/clustalw/).ac.uk/clustalw/
The completeness and specificity of the sequence databases directly affect the searching results.
Nucleotide BLAST was performed to check the specificity of the sequences.
Primers were designed to confirm X and Y specificity of the sequences.
There were no cross-superfamily connections, highlighting the specificity of the sequence search through the MQ approach.
Then, BLAST resources in NCBI were used to assess the degree of specificity of the sequence recognition for these siRNAs.
Thus, we found that the 10AA code failed when we tried to infer the specificity of the sequences we examined.
In this study, we compare the known binding sites of the DCC with oligonucleotide profiles that measure the specificity of the sequences of the D. melanogaster X chromosome.
