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The resulting overall calcium response in the cell, deduced from the combination of the nuclear and the cytosolic calcium response might contribute to the specificity of the final response to a specific stimulus.
By using restriction enzyme digestion and subsequent electrophoresis, specific DNA fingerprints were predicted and confirmed for each subfamily, thus increasing the specificity of the final result compared to stand-alone RT-PCR.
The specificity of the final immunoreagent was determined by immunoblotting against the full range of AMPA receptor subunit CTDs.
The specificity of the final products was strongly controlled by the first single strand amplification.
Specificity of the final choice of antibody for XIAP was evaluated by analysing a panel of cell lines including clone X-G4.
The algorithm also allows users to specify options (such as the level of phosphorylation site mismatch tolerance and the maximum length between two entities to be considered) for adjusting the complexity and the specificity of the final prior network.
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ROC analysis was used for definition of the optimal diagnostic cut-offs and description of their sensitivity and specificity for the final diagnosis.
A receiver operating characteristic (ROC) curve was used to measure sensitivity and specificity of the fitted final model at 95% CI and P < 0.05.
Serum containing the antibody was used for biochemical analyses to determine specificity and efficacy of the final product.
Sensitivity and specificity of the immunoassay versus final diagnosis of active tuberculosis were 89% (24 of 27) and 80% (12 of 15) respectively.
A separate search was performed for these 5 tests to compare their accuracy in terms of sensitivity and specificity, using the final histopathologic diagnosis as a reference.
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