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In our study, neither PCR amplification products nor fluorescence signals were observed in the control condition, indicating high specificity of the established real-time PCR assay.
No false positive amplification was observed, indicating the high specificity of the established LAMP assays.
Specificity of the established Rickettsia real-time pyrosequencing was validated by use of positive control plasmids containing the gltA target sequence of the individual Rickettsia species of interest.
The serial twofold dilutions of the soluble CD28-Fc, PD-L1-Fc, and PD-L2-Fc proteins starting from 100 ng/ml were detected by the ELISA to assess the specificity of the established system.
To examine the sensitivity and specificity of the established antibodies in immunoblot analysis and immunoprecipitation, the TMEPAI/V5 or C18ORF1/V5 expression plasmids were transfected into COS7 cells (5 × 10 cells/6-cm dish) using FuGENE 6 (Roche Applied Science).
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The antisera were used for the specificity analysis of the established cELISA.
Using a panel of 60 swine biological samples (feces, intestinal contents, and sera) characterized by PEDV, PDCoV and transmissible gastroenteritis virus-specific RT-PCR assays, we demonstrated that sensitivity and specificity of the newly established panCoV RT-PCR assay were 100%.
Protocol and specificity of the previously established immunoisolations of calsyntenin-1, syntaxin13, Rab11, and Rab5 organelles were reported previously (Steuble et al., 2010).
Additionally, a study analyzed the sensitivity and specificity of the 3 established real-time PCR methods using primers and TaqMan probes targeting the IS711, bcsp31 and per genes, and it also compared their efficiencies for the detection of the Brucella genus.
In colon carcinomas, hERG mRNA was a more sensitive and more specific indicator for malignancy (100% sensitivity and specificity; n=23) than mRNA of the established tumor markers CEA (sensitivity=94.4% n=18), CK19 (sensitivity=77.8% n=18), or CK20 (sensitivity=94.4% n=18).
The forced degradation study was planned to investigate specificity and stability-indicating property of the established method.
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