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We describe a new software program, SLICSel, for designing specific oligonucleotide probes for microbial diagnostics using nearest-neighbor thermodynamic modeling and evaluate SLICSel by testing the specificity of the designed tmRNA specific probes.
The concept of optoacoustic imaging using home-made functionalized gold nanoparticles coupled to an antibody targeting PSMA (prostate specific membrane antigen) was evaluated on different cancer cell lines to demonstrate the specificity of the designed platform.
The specificity of the designed aptamer was investigated by AFB1.
The specificity of the designed primers was evaluated in three steps.
The specificity of the designed oligonucleotides was evaluated, furthermore the optimal spotting concentration was determined.
Specificity of the designed IT primers was tested by sequence analysis of amplified IT fragments in a randomly chosen locus.
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The size and hydrophobicity of the involved amino acids were rationally changed, and the substrate specificities of the designed d-Hyd mutants were investigated.
Phylum-specific and subgroup-specific primers were designed from the consensus sequences of the phylotype sequences, and the specificities of the designed primers were evaluated both in silico and empirically for coverage and tolerance.
The gene-specificity of the designed primer pairs was validated in four ways.
Specificities of the designed amplicons were tested in silico using BLASTn search of public databases [ 53].
Both methods confirmed the high specificity of the primers designed.
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