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Possible explanations for substrate specificity of the biosensor were discussed.
Simultaneously, to further improve the sensitivity and specificity of the biosensor, P-ERCA assay was introduced.
The sensor shows negligible response to mismatched proteins such as Immunoglobulin G (IgG), confirming the specificity of the biosensor.
Compared with bacteria culture method, the detection sensitivity and specificity of the biosensor system were 94.12% and 90.91%, respectively.
The specificity of the biosensor was tested against four different foodborne associated bacteria (Escherichia coli, Salmonella, Shigella flexnerii and Staphylococcus aureus).
In cell-impedance measurement, non-transfected HEK-293 cells were not responsive to salicin, which proved the specificity of the biosensor.
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To improve the specificity of the biosensors, 6-mercapto-1-hexanol (MCH) as a blocking reagent after capture probe immobilization was employed.
The specificity of this biosensor assay was 100% when it was tested with 13 representative pathogenic Leptospira species, 2 intermediate Leptospira species, 1 non-pathogenic Leptospira species and 28 other bacteria species.
The specificity of the electrochemical biosensor was then tested using non-complementary, or mismatch short DNA sequences.
These results demonstrate the sensitivity and specificity of the tyrosinase biosensor designed for in vitro detection of TK activity, which is known to be involved in several diseases.
The good linearity of the calibration curves for PBS and lysate solutions at low Sur concentrations confirm the high specificity of the proposed biosensor and good discrimination against nonspecific interactions with lysate components.
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