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The specificity of the bioelectrode towards specific cTnI was examined by monitoring the responses from multiple controls such as native normal human serum, non-specific biomarkers C-reactive protein (CRP), myoglobin (cMb) spiked in human serum, and a negative control with a native electrode (PPy-PPa/G-CNTs/GCE) without anti-cTnI immobilization.
These results predicted a good specificity of the bioelectrode for cTnI.
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The comparative response of the bioelectrode with multiple controls with respect to the specific cTnI under identical condition is shown in Fig. 4d.
Figure 4c shows the calibration curve of the bioelectrode.
c Concentration-dependent calibration curve of the bioelectrode.
Figure 4 shows the Nyquist and the Bode plot of the bioelectrode with different cTnI concentrations.
The stepwise fabrication of the bioelectrode is schematically represented in Fig. 1.
The reproducibility of the bioelectrode was evaluated in terms of relative standard deviation measured at individual cTnI concentration on three different bioelectrodes prepared in the same manner under identical conditions.
The response characteristics and optimization of the bioelectrode design were evaluated.
The sensitivity and detection limit of the bioelectrode 2 for nitrate are 94.7 mA M−1 cm−2 and 0.5 μM, respectively.
The stability of the bioelectrode in solution was also investigated by taking repeated measurements (5 6 times) with a fixed concentration (1.0 ng mL−1) of cTnI sample.
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