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The use of locked nucleic acid (LNA) probes and primers potentially improves sensitivity and specificity of quantitative PCR (qPCR) assays.
Now sensitivity and specificity of quantitative HCVcAg assay has been increasingly improved.
The specificity of quantitative RT-PCR was confirmed by sequencing the PCR products.
The specificity of quantitative analysis of amyloid PET imaging is comparable to that of other methods.
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A melting curve was obtained from a first step starting from 60 to 95°C, to control specificities of quantitative PCR reaction for each primer pair.
The quantitative RT PCR method using multiple markers reportedly improved the sensitivity and specificity of the quantitative RT PCR method (Guller et al, 2002; Timar et al, 2002; Osaka et al, 2004; Fujita et al, 2006).
Although the high sensitivity and specificity of the quantitative, real-time, polymerase chain reaction are justified [35], the limitations of this technique are the sensitive nature of the work and the cost of the chemicals and equipment.
To evaluate the sensitivity and specificity of calcaneal quantitative ultrasound (QUS) measurements for identifying osteoporosis determined by dual-energy x-ray absorptiometry (DXA) at the hip in a spinal cord injury (SCI) population.
To estimate specificity of labeling, quantitative evaluations were carried out on ultra-thin sections.
A melting curve analysis was performed after each analysis to confirm the specificity of the quantitative PCR (qPCR).
However, the specificity of the quantitative PCR for cccDNA is always a concern because a vast excess of RC-DNA is present in infected hepatocytes.
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