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Eukaryotic DNA polymerases-α, -β and -δ differ in their frequency and specificity of producing frameshift errors, polymerase-β being the least accurate enzyme (9).
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To determine in vivo specificity of produced CTLs, magnetically labeled CTLs sensitized to U251 cells were intravenously injected in mice bearing either subcutaneous MBA-MD-231 breast cancer (n = 2) or U251 glioma (n-2).
Moreover, it is intrinsically of interest given its wide substrate specificity; it is capable of producing hydrogen from a variety of six and five carbon sugars and sugar derivatives [ 16].
However, it was reported that the introducing of a "spacer group" between the drug molecule and the carrier protein usually increases the specificity of the produced antibody [34].
Substitution during conjugation can adversely affect the activity and specificity of antibodies produced, and it also can determine the analytical strategies of protein conjugates employed.
However, LDH release studies demonstrated the specificity of the produced CTLs against U251 cells.
These data strongly indicate the immunological specificity of the produced CTLs for U-251 cells (Figure 3B).
In addition to the study of conservation patterns in FAE sequences, we have examined the patterns of conservation and variation in enzyme function by analyzing the substrate specificity of FAEs produced by a large number of ascomycota.
Since there are no other reports on strain characterization based on the substrate specificity of a produced FAEs mixture, we can compare our findings only with studies on isolated FAEs.
To determine the cytotoxic specificity of the produced CTLs to U251 cells, 200k CTLs (sensitized to U251 cell lysate) were co-cultured overnight with U251 (100k) or human breast cancer cell (MBA-MD-231, 100k) and the released lactate dehydrogenase (LDH) was determined by a commercially available membrane integrity assay kit (Cyto Tox-ONE, Promega corp, WI).
This confirms the specificity of antibodies produced by the present study.
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