Sentence examples for specificity of polymerase from inspiring English sources

The PCR products were electrophoresed on a 1 % agarose gel and visualized upon staining with ethidium bromide to detect the specificity of polymerase chain reaction.

RpoS alters the specificity of RNA polymerase, directing transcription for a suite of genes involved in starvation response [ 20].

Moreover, we found that the low specificity of RNA polymerase II TSSs selection generates, for 47% of the expressed genes, NMD-sensitive transcript isoforms carrying uORFs or starting downstream of the ATG START codon.

Thus, using pol β allows us to examine mechanisms of the microsatellite mutation process in a manner that will not be biased by the inherent error specificity of the polymerase.

To further explore the apparent change in promoter specificity of this polymerase, we carried out an additional screen in which R3 17 was again cloned adjacent to the library of promoter mutations, and replica-plated colonies that grew poorly or not at all upon IPTG induction (indicating polymerase overproduction) were picked and sequenced.

Based on our studies of replicative, repair and specialized polymerases, it is unlikely that the observed directionality bias favoring microsatellite expansion in cells reflects the inherent error specificity of DNA polymerases.

Background: For many diagnostic applications, the specificity and sensitivity of polymerase chain reaction (PCR) is markedly enhanced by applying two rounds of PCR with nested or semi-nested pairs of primers.

This may be a viral strategy, where the low specificity of the virus polymerase generates frequent mismatches and a high rate of mutations.

Similar studies have been used to alter the promoter specificity of T7 RNA polymerase.

In row 2, the non-specific hybridization can't bring incorporation of Cy5-dCTP into the DNA due to the specificity of Taq DNA Polymerase.

Five of the six nuclear-encoded sigma factors that modulate the specificity of plastidic RNA polymerase are present in Regulon 2 (SIG1-SIG4 and SIG6 [ 68]).