Exact(13)
We were also interested in analyzing some of the most highly methylated CRC genes reported in the literature, recently reviewed in Kim et al. From this gene list, we selected candidates that had been analyzed using real-time quantitative methylation-specific PCR (qMSP) with resulting sensitivity and specificity measurements in tissue samples of at least 80%and90%0%, respectively.
For binding and specificity measurements, 10 nM 125I-Fab or 111In-Fab was added to each dish and incubated for 2 to 24 h.
The sensitivity and specificity measurements are around 90%.
Exact specificity measurements are in Table S3.
But as mentioned previously, a real genome already has its own HTs that could interfere with the specificity measurements, as they would likely be detected by the methods.
Using accurate binding specificity measurements together with principled TF binding prediction methods can provide a competitive alternative to traditional condition specific ChIP-chip experiments, especially when TF binding prediction incorporates multiple additional data sources.
Similar(47)
To determine the sensitivity and specificity of measurements of the retinal nerve fiber layer (RNFL) using the StratusOCT in glaucoma subjects with visual field (VF) defects.
To evaluate the sensitivity and specificity of measurements of retinal nerve fiber layer (RNFL) thickness in early to moderate glaucoma using Stratus time-domain and Cirrus spectral-domain optical coherence tomography (OCT; Carl Zeiss Meditec, Dublin, CA).
All statistics including linear regression and interpolation for the ELISA assay were performed using GraphPad Prism 5. To compare sensitivity and specificity of measurements in the tumour cohort compared to the benign cohort receiver-operator curves (ROC) were performed.
The results are evaluated in terms of accuracy (Accu), sensitivity (Sens), and specificity (Spec) measurements [ 16].
The blood measurement of chlorpyrifos would offer more chemical specificity than measurement of urinary TCPY.
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