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The selected antibody DNA can be further manipulated for high-level expression, post-translation modification, and/or affinity and specificity improvement to suit their particular applications.
As a trade-off between specificity improvement and sensitivity loss, biosensors were designed to perform indirect immunoassays with amperometric detection using tailor-made chimeric receptors to react with the analyte, specific anti-Trypanosoma cruzi immunoglobulin G (IgG).
The most significant specificity improvement came from mutant H358A.
In addition, we showed that the presence of SialylLewisx and core fucosylation structures could facilitate the specificity improvement of CEA for cancer detection.
An ~8% gene-level sensitivity and ~6% specificity improvement to predictions is made in stage 1, where these annotations are replaced by GHMM predicted genes.
The latter indicates that by using CAD and increasing the biopsy threshold from PIRADS 3 to 4 almost no loss in detection of aggressive lesions (sensitivity 0.99 vs. 0.98) occurs, while a specificity improvement is obtained (0.59 vs. 0.26), reducing unnecessary biopsies.
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The methodology, accuracy, and specificity improvements incorporated into Prodigal have been described [ 11].
Two indices may be used to assess the performance of the OxyGene platform: the specificity (improvements in original annotations, see above) and the sensitivity (discovery of missed features).
In order to achieve higher sensitivity and specificity, major improvements are needed.
MOS devices layout specificity towards performance improvement, namely breakdown, parasitic effects and degradation, will be emphasized.
In addition, we do observe higher probe specificity and other improvement in the recent yeast2.0 chips.
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