However, studying retrospective data on ER status has the disadvantage that the assays used to establish ER status, and hence their sensitivity and specificity have changed over time.
The possibility that DNA binding specificity of CtrA has changed in these bacteria is an intriguing hypothesis.
However, NSD1 and WHSC1L1 HMTs have been proven to carry totally different specificities [23], [77], suggesting that at least one of them has changed its specificity during evolution, which support the second possibility described above.
Since their introduction into humans, the receptor-binding specificity of human H3N2 viruses has changed continuously [12], [34], [35], [36], [37], [38].
These observations suggest that specificity of the receptor members has changed during evolution and may explain the failure to identify conserved ligands.
This allowed us to reveal how DNA specificity would have changed along any direct trajectory from AncSR1 to AncSR1+RH.
Thus, we have changed the specificity of the FHA1 domain and demonstrated that engineering phosphopeptide-binding domains is an attractive avenue for generating new anti-phosphopeptide binding specificities in vitro by phage display.
In addition, it is still an open question if factors have changed host specificity of the hybrid.
We have changed the target specificity of EcoDam from GATC to GATT by directed evolution, combining different random mutagenesis methods with restriction protection at GATT sites for selection and screening.
Therefore, we hypothesized that either mutations in the erm gene promoter region have upregulated methylase expression or that mutations in the coding region have changed the methylase specificity to include the additional binding sites of telithromycin.
