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The delta-derived ribozyme cleaved substrate RNA at a specific site, and the sequence specificity could be altered with mutations in the region of the ribozyme proposed to base pair with the substrate.
In the case of malaria, which is transmitted only by anopheline mosquitoes, maximal specificity could be achieved through identification of immunogenic proteins specific to the Anopheles genus.
Specificity could be achieved by differential interactions with protein cofactors.
By increasing HCO3− concentration, the reaction specificity could be redirected to malate production.
The variation in recognition sequence specificity could be used to tune the number of genes targeted by MazF.
However, a lack of effectiveness and/or specificity could be a major drawback for research or clinical applications.
This specificity could be achieved by use of different regulatory elements by the homeoproteins or by use of the same elements in different ways.
This lack of specificity could be attributed to the SR protein family which is known to have redundant activity for some substrates in vitro and in vivo53,54.
Experiments were done to find whether the specificity could be exploited to direct the hydroxylation of multiply substituted aromatic substrates and thereby produce novel catechols.
This high sensitivity and specificity could be attributed to not only the exponential amplification and excellent discrimination of LCR but also the employment of SA-MPs.
Binding specificity could be manipulated experimentally through various display technologies that allow us to select desired binders from a large pool of candidate protein sequences.
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