Furthermore, as a specificity control, preincubation with Maackia amurensis lectin, which specifically binds to SA2,3Gal terminated oligosaccharides, was performed.
In addition, specificity control for the staining was included using cells treated with 500 nmol/L H2O2 solution.
Although this may suggest sequence-specific interference, inhibitory effect on viral replication was also observed in cells transfected with a plasmid expressing pre-miRNA targeting β-galactosidase gene (miR-LacZ) that served as a specificity control.
For specificity control, a sense probe was used.
Laminin coating was used as matrix specificity control for differentiation.
Intestinal tissue from CCR9−/− and CCL25−/− animals was used as a specificity control (Figure 1A).
As a specificity control, we used IL-20, a related IL-10-type cytokine.
Although the result was negative, it served as IF specificity control (not shown).
Leptin receptor was used as Fc protein and binding specificity control.
The corresponding isotype control antibody (R&D Systems) was applied for specificity control.
Anti-isotype antibodies (BD Biosciences) were used in parallel, for specificity control.
