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scFv antibodies chosen from the resulting panel were purified and their specificity confirmed by ELISA.
The use of reactivated memory T cells of an irrelevant specificity confirmed this hypothesis.
Primers were designed using the ABI Primer Express software and their specificity confirmed using NCBI BLAST module.
As in the embryo, the stain is distinctly nuclear (Fig. 1A), and its specificity confirmed by a control with 2° antibody, but no 1° antibody (Fig. 1B).
Reactions generated single PCR products that were identified by sequencing (SUPAMAC, Sydney, Australia) and specificity confirmed by BLAST searches.
Specific primers for quantification were designed using the Universal Probe Library Assay Design Center (Roche, Welwyn Garden City, UK) and target specificity confirmed with Primer-blast (NCBI).
Similar(44)
Comparisons to a previously constructed model of human TAP specificity confirms the well-established differences for peptide substrates with positively charged C-termini.
The comparison of HIV-positive patients and noninfected controls for HLA class I specificities confirmed the previously described association of A2/A6802 supertype with resistance to HIV.
Primers for the putative DMXAA-regulated mRNAs CCL3, CCL4 and CCL7 and 'housekeeping controls' YWHAZ and PPIA were designed with a 3′ base difference between species and their species-specificity confirmed using RT PCR of control RNAs from mouse and human cells.
Specificity was confirmed with competition experiments.
Assay specificity was confirmed by capillary electrophoresis and DNA sequencing.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com