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To understand how kinase phosphorylation site specificity changes in evolution from a structural standpoint, we sought to identify specific residues in the kinase catalytic domain that mediate +1 specificity.
A comparison of these structures, in combination with the specificity profiles of the variants, reveals subtle differences that explain the details of the specificity changes.
Finally, we report successful prediction of several amino acid specificity changes from blind tests in the DREAM4 peptide recognition domain specificity prediction challenge.
We also find that we can computationally recapitulate specificity changes upon mutation, a key test for successful forward design of protein protein interface specificity.
Forty-eight hours post-injection, tissue specificity changes (Fig. 5) from the difference in the rank order of concentration, i.e., liver > kidney > lung > spleen for the PPN and spleen > liver > kidney ≥ lung for Pax per se.
Societal contexts and cultural specificity changes, but the youthful soul does not.
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This specificity change is complete and shows little preference for any other specific sequences.
The flexibility of the described design allows for sensor specificity change through altering the specificity of the aptamer.
BtsI-1 is the first REase other than Type IIG with a complete specificity change.
This is the first successful case of a specificity change among this restriction endonuclease type.
The reason that BtsI-1 can survive after the specificity change is because we expressed the BtsIA and BtsIB separately.
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