However, these methods can only provide the overall interaction pattern or internal motion of purified protein complexes, and have many limitations such as low specificity, high background or false positives (Syafrizayanti et al., 2014).
Despite these conditions, its high specificity, low background signal, high signal-to-noise ratio and the ability to study gene expression over the lifetime of the animal are reasons why this technology is used extensively for in vivo imaging studies.
The best LAP possesses the highest sensitivity (>50% expression) and the best specificity (<0.1% background).
This was either due to limited sensitivity or reduced specificity (high background or multiple bands on the Western blot).
Control sections incubated in the absence of primary or secondary antibody were also processed and evaluated for specificity or background staining levels.
In addition, we tested the specificity and background of this antibody on meiotic tissue from mutant Drosophila females that were either defective in DSB formation or defective in the ability to repair meiotically induced DSBs.
Note, however, that our goal is not de novo motif discovery but probabilistic inference of TF binding, given some a priori information about TF binding specificities and background sequence properties.
The sensitivity (100 ng) and specificity (high-background staining) of Coomassie may remain a problem despite the improved colloidal version; however, it is cost-effective, relatively simple to use and offers good compatibility with MS [ 11, 80- 84].
Women wanting to escape violence at home and rebuild their lives thereafter are more likely to do so if they have an organisation that, as Gabriela Quevedo of LAWA puts it, "understands the specificity of their background".
In practice, the preparation of such conjugates is challenging, and sometimes the produced conjugates do not have the required specificity and produce background noise [20].
