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The specificity assay showed it was specific for T. sergenti whilst the LAMP was able to detect a parasitemia level of 0.000002% which was more sensitive than conventional PCR.
Two parallel methods were used to independently determine relative DNA sequence-specific binding affinities: high-throughput microwell-based TF DNA-binding specificity assay (Hallikas and Taipale, 2006; Hallikas et al, 2006) and protein-binding microarrays (PBMs Bergerr et al, 2006).
Using a quantitative specificity assay, we found that these Fabs are highly specific, possibly due to the alternate codon design we used to avoid consecutive arginines in the Fab interface.
In the IPC specificity assay no signal of fluorescence was obtained using primers and probe specific for rodent RNA amplification.
The specificity assay for anti-rituximab antibodies was carried out in a manner identical to that used for the anti-InO specificity assay.
A specificity assay showed no cross-reactions with other common RNA viruses.
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The results of specificity assays revealed no non-specific products, and indicated the presence of the expected amplicon for each gene.
Another 10 heterologous parasites were selected for specificity assays.
Analytical performance and specificity assays were carried out using human serum and different proteins.
Indeed, additional genome-wide specificity assays are sorely needed for in-depth and accurate investigation of the in vivo specificity of base editors.
Samples with a screening OD value greater than or equal to the cut point OD were reassayed in concurrent titer and specificity assays.
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