Exact(4)
The five species of Phyllanthus were subjected to amplification for testing the specificity and discrimination power of the designed primers derived from rbcL and trnL regions.
Forward LNA primers (Exiqon, Denmark) were used to amplify mmu-miR-146b, -29b and 29c in order to increase specificity and discrimination that were not obtained with native DNA primers.
DNA polymerase fidelity, specificity, and discrimination are relative kinetic terms used to describe the probability of a polymerase producing a base substitution error (i.e., mismatch).
Long oligonucleotide probes generated for this novel C. melo microarray (60 mers) provide superior signal-to-noise ratio, increased sensitivity, specificity, and discrimination.
Similar(56)
Specificity for and discrimination between target sites, effected primarily by amino acid residues that contact DNA, can be modulated by those that do not interact with DNA or with a DNA-contacting residue.
Here we have assessed the specificity, sensitivity and discrimination of six different primer pairs, three targeting nuclear and three mitochondrial regions, for use in identification of Australian basidiomycete fungi from Eucalyptus forests by matching PCR-RFLP patterns to morphologically defined species.
Sensors that rely on non-specific hydrophobic effects are first considered before looking at the use of specific, directed intermolecular interactions to achieve greater specificity and chiral discrimination.
Schache AG, Liloglou T, Risk JM, Filia A, Jones TM, Sheard J, Woolgar JA, Helliwell TR, Triantafyllou A, Robinson M, Sloan P, Harvey-Woodworth C, Sisson D, Shaw RJ (2011) Evaluation of human papilloma virus diagnostic testing in oropharyngeal squamous cell carcinoma: sensitivity, specificity, and prognostic discrimination.
Less expensive than TaqMan-based assays, SYBR® green assays theoretically allow for species specificity and species discrimination based on a melting curve and the Tm value of the amplicon.
The use of shorter length MGB and LNA probes is advantageous, typically confering stability, target specificity, greater sensitivity and discrimination for the target gene [ 19].
ROC analysis indicated that the area under the ROC curve (AUC) is 0.893 for NeuroD6 and 0.474 for NeuroD1, and the levels of sensitivity and specificity for discrimination between AD and NC are acceptable for NeuroD6 (P = 2.494 E − 04) but unacceptable for NeuroD1 (P = 0.811) (Supplementary Figure 1).
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