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Positive identifications had to reach the following specifications: sequence coverage of at least 30% and a MOWSE-score of at least 49.
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Sequence specifications for breast DCE-MRI should consider the effect of FA and k-space sampling schemes, particularly for studies across multiple systems or institutions.
It thus is the first photoreceptor cell poised for disappearance by early termination of the ommatidial cell fate specification sequence.
Each tool is used in a specific sequence, and sometimes made to order by clipping, peeling, stripping or splitting the wood to the desired specifications.
All positive amplicons were purified with the Roche High Pure PCR Template Purification kit (according to manufacturer specifications), and sequenced with each of the PCR primers in separate reactions.
Assay specifications and sequences are summarized in Table S3.
Our framework allows the specification of sequence homology thresholds, which enable the user to define the relationship between sequence similarity and function.
Detailed algorithms and specifications for sequencing analysis including read filtering, mapping, and differential expression calculations are provided in the Supplementary Methods and Supplementary Fig S14.
Even among the yeasts, modes of centromere inheritance vary, from complete genetic specification, as in Saccharomyces cerevisiae, to genetic specification without clear sequence specificity, as in Schizosaccharomyces pombe (Polizzi and Clarke 1991), to complete epigenetic inheritance, as in Candida albicans (Ketel et al. 2009).
Specification of sequences obtained from NCBI GenBank.
Table 1 Primer specifications Primer Target Sequence (5'-3') Reference Pla46 F Planctomycetales GGATTAGGCATGCAAGTC Neef et al. 1998 616 F Most bacteria AGAGTTTGATYMTGGCTCAG Juretschko et al. 1998 EubI R Most bacteria GCTGCCTCCCGTAGGAGT Amann et al. 1990 EubII R Planctomycetales GCAGCCACCCGTAGGTGT Daims et al. 1999 EubIII R Verrucomicrobiales GCTGCCACCCGTAGGTGT Daims et al. 1999.
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