Sentence examples for specification in vivo from inspiring English sources
Because stella is specifically expressed in PGCs during PGC specification in vivo [18], we wanted to determine whether the derived GFP-positive cells from a day 7 attachment culture (Att+) and a day 4 EB culture (EB+) contained PGCs (Fig. 2A).
However, significant difficulties exist with regard to research into the process of PGC specification in vivo.
Q61L/H We describe miRNA expression changes that occur during trophectoderm specification and validate that our in vitro system faithfully recapitulates trophectoderm specification in vivo.
Recently a systematic analysis of single cell expression has revealed the gene expression dynamics in germ-line cells during PGC specification in vivo [17] and indicated differential expression patterns between ES cells and PGCs, such as their expression of Eras, T, and Fgf8.
Interestingly, modulation of ΔNp63 expression in zebrafish embryos has demonstrated its role in early dorsal/ventral ectodermal patterning as well as neural/epidermal specification: in vivo inhibition of p63 was shown to prevent skin formation, while its exogenous expression promoted epidermal specification [21], [22].
10.7554/eLife.00806.020 Figure 8. Anterior specification in vivo requires PI3K-dependent ECM.
Furthermore, Ras-MAPK signaling induces Cdx2 expression, and Erk2 is apically localized in the 8-cell embryo; this suggests an early role for Ras-MAPK signaling in specification of TE in vivo and indicates that our in vitro differentiation system faithfully recapitulates embryonic TE specification [36].
Exogenous factors, such as BMPs and Wnts are well-known modulators of NC specification and migration in vivo [20], [23], [52].
However the key factor of T lineage specification and commitment in vivo is to be understood.
The requirement of these factors for specification of pluripotency in vivo and maintenance in vitro and their expression kinetics during pre-implantation development have been reviewed recently [ 4] and will not be recounted in detail here.
Thus, this distribution pattern of dorsal cortical subtype-specific neurons resembles in vivo regional sub-specification, indicating these organoids derived from our culture system could efficiently model neocortex organization in vivo.
