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Sequential specification function method is used to solve the IHCP and estimate the space-variable convective heat transfer coefficient.
It is unclear whether Foxp3 exerts its Treg lineage specification function through active modification of the chromatin landscape and establishment of new enhancers or by exploiting a pre-existing enhancer landscape.
We therefore challenged the robustness of the MN pool specification function mediated by the Met signalling-switch mutants, by genetically lowering Pea3 expression levels.
Thus, the robustness of the cell fate specification function triggered by the Met RTK relies on a complex signalling circuit possibly composed of a combination of distinct transcription factors, the activity of which is modulated qualitatively and/or quantitatively by upstream signalling cascades.
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These results highlight that DmExd and NvPbx are functionally equivalent, at least for specification functions in the Drosophila epidermis.
The hierarchy and multiple additional design features of these networks act to produce Boolean regulatory state specification functions at upstream phases of development of the body plan.
Our data suggest that Platynereis bHLH genes have both proneural and neuronal specification functions, in a way more akin to the vertebrate situation than to that of Drosophila.
In insects, such as Drosophila, the specification functions of neural bHLH genes (and even some genes such as Olig) have been lost.
Our analysis suggests that the Platynereis bHLH genes have both proneural and neuronal specification functions, in a way more akin to the vertebrate situation than to that of Drosophila.
The significance of this observation lies in the critical subtype-specification function of Ascl1 in p3[5HT] and p3[V3] progenitors, which is separable from its neurogenic properties [ 11, 12].
In mammals, a requirement for Ascl1 in serotonergic differentiation has previously been demonstrated, including for the expression of 5HT itself, and this subtype-specification function cannot be substituted by other proneural genes (Jacob et al., 2009; Parras et al., 2002; Pattyn et al., 2004).
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