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As shown in Figure 1E, RPC1 was specifically recovered from extracts supplemented with Maf1 (compare lanes 2 and 3).
Tud was specifically recovered in Vas-complexes when a cocktail of RNase inhibitors was added during extraction of the ovarian proteins (Fig. 5D).
We also specifically recovered four subunits of SWI/SNF complexes (Table 1): SMalso2/BAF190 (also known as Brahma/BRM), and three Brahma-associated factors (regrettably, also known as BAFs): SMARCC2/BAF170, SMARCE1/BAF57 and the actin-related protein BAF53A/ACTL6A.
It has demonstrated the effectiveness of pyrosequencing in rapidly capturing large sections of the Mytilus transcriptome and shown that bar-coding via MID tags can increase productivity, allowing sequences from different tissues to be determined simultaneously but then specifically recovered at the bioinformatics stage.
As shown in Figure 2A, several species were specifically recovered in the anti-cystatin F precipitates.
The affinity purification therefore specifically recovered autoantibodies recognizing the SRP54N domain while the unbound fraction was completely depleted of this activity.
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Further, we show the resulting coverage profiles to match previous data for the actively transcribed gene associated (H3K4me3) and repressed gene associated (H3K27me3) histone marks, and to specifically recover known myeloid specific CEBPA target regions.
We asked whether some of the more variable components of flagella could be used as queries that would specifically recover these core genes.
Specifically, we recovered two monophyletic groups, each containing taxa with low, moderate, and high swimming performance.
Specifically, 454 recovered 114.58% and 191.59% more proteins than Shotgun and Fosmid, respectively.
Specifically, we recovered three proteobacteria species, Saccharophagus degradans, Cellvibrio japonicus, Stigmatella aurantica, and one bacteroidete species, Microscilla marina, nested among green plant sequences with strong statistical support.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com