To visualize Purkinje cell dendritic arbors, parasagittal cryosections (12 μm) were cut from both cerebellar hemispheres, starting 1 mm from the midline, and reacted with antibody specific for calbindin-D28K (Sigma, St . Louis MO), which specifically labels Purkinje cells (Christakos et al. 1987).
To understand the progressive enlargement of eGFP-KRASG12D-positive masses, we assessed cell proliferation by using an antibody that specifically labels the PCNA (proliferating cell nuclear antigen) protein, a specific marker for cells in S phase of the cell cycle.
As the eGFP label had been eliminated by decalcification of the tissues, we also labeled sections with HuNu, an antibody that specifically labels human nuclei and not those of mice. Figure 3A,B illustrates HuNu-labeled cells in the femoral growth plate that were associated with human-specific collagen II staining (Figure 3E,F).
He specifically labels Archytas' solution as dysmêchana, "hardly mechanical".
For this purpose, tissue samples were stained with an anti-GFAP antibody which specifically labels astrocytes.
An answer became possible about 2 years ago, when researchers developed the first marker that specifically labels lymph vessels, making them easy to spot under a microscope.
5 Uses a single CRF to identify SYSTEMATIC, TRIVIAL, FAMILY, ABBREVIATION, FORMULA and IDENTIFIER entities and specifically labels each class of entity.
While our results indicate that the knock-in of mCherry into the Nestin locus recapitulates its endogenous expression, we next sought to define whether mCherry specifically labels neural progenitors.
To further assay mitochondrial mass, we used NAO, which specifically labels the mitochondrial phospholipid cardiolipin, and is commonly used as a good readout to estimate mitochondrial mass.
To identify stable microtubules, we used a monoclonal antibody directed against acetylated-tubulin [19], which specifically labels stabilized microtubules in cilia and axons [19], [20].
Consequently, a synaptotagmin 1-GFP fusion construct (SytGFP) was developed as a synaptic vesicle marker that specifically labels presynaptic sites [52].
