To specifically label cholinergic neurons, ChAT-Cre mice were crossed with tdTomato reporter line (The Jackson Laboratory, stock number 007909).
A family of reagents has been developed that specifically label deubiquitylating enzymes (DUBs) and facilitate characterization of their inhibitors.
Overall these results indicated that SBA can be utilized to specifically label RSV G in live cells without altering viral protein expression and localization.
Overall, these results indicate that SBA can be used to specifically label RSV G, supporting previous lectin-binding studies to RSV glycoproteins34.
Katherine M. Clark (D-Mass). and Elizabeth Esty (D-Conn). referenced the "terror" in Las Vegas but didn't specifically label the attack as such.
Here, we utilize rapid 4-thiouracil (4-TU) incorporation via pyrimidine salvage to specifically label, capture, and quantify newly-synthesized RNA transcripts at every hour throughout the IDC.
Most notably for gene delivery, they have been used to specifically label plasmids and act as adapters to link synthetic peptides or ligands to the DNA.
To specifically label VTA DA neurons, midbrain sections were incubated (30 min) in PBS-BT (phosphate-buffered saline (PBS), 0.5% bovine serum albumin, 0.1% Triton X-100) with 10% normal goat serum (NGS).
While we only describe the use of fluorescent SBA specifically label RSV G, fluorescent lectins can be screened against the glycoproteins of other viruses, possibly allowing for the study of live glycoprotein dynamics in a variety of pathogens.
The yAP is not recognized by BirA, and thus, we were able to specifically label AP and yAP fusion proteins coexpressed in the same cell with differently colored QDs.
In Section III.C we explain how we specifically label our microfabricated structures, and in Section III.D we present how these functionalized microfabricated structures are incorporated into assays, with a discussion of the details of the assays themselves.
