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For each experiment, a set of experiment-specific parameters, such as the effect of transfection on production rates or the activation level in growing cells, was specifically incorporated to reflect the experimental setup and conditions.
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However, recently we found that when the unnatural amino acid pAcF is site-specifically incorporated to produce a mutant recombinant fibroblast growth factor 21 (rhFGF-21) in E. coli, it is misincorporated for proline with roughly 0.5% frequency/proline.
We previously reported that a yeast-derived recombinant fusion protein comprising α2AP residues 13-42 linked to human serum albumin (HSA) weakened in vitro clots but failed to become specifically incorporated into in vivo clots.
This type of seeding process was specifically incorporated because the optimal solution to a problem always lies between the maximum serial and parallel implementation.
Figure 2 shows the 32+ charge state of sfGFP and clearly illustrates mass shifts corresponding to the incorporation of each of the specifically incorporated pAzF residues.
Alternative promoters existed in 7,708 genes, of which 57% transcribed a first exon that was not exclusive to the isoform and 42% specifically incorporated a DAlFE into the isoform (Figure 1C).
Under single turnover conditions (e.g., 200 nM DinB and 100 nM DNA), DinB specifically incorporated dCTP opposite template-dG: we did not detect incorporation of dATP, dGTP, or dTTP (Table 3).
For example, we have not specifically incorporated phenotype information when selecting reference haplotypes, so the selected reference panel is not guaranteed to include the individuals with traits of interest.
Although judicial review had been relatively uncommon before World War II, by the early 21st century more than 100 countries had specifically incorporated judicial review into their constitutions.
At the Peabody Museum, an exhibit was developed whose primary theme concerned a medical topic (two vector-borne diseases) and then specifically incorporated Darwinian evolution into the narrative.
If musicians specifically incorporated interpreters into their act they would demonstrate a true appreciation for deaf fans by guaranteeing a performance up to the artists' standard.
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