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This method proved to be better than a Rnase H digestion protocol, in which we tried to remove the poly(A) tail from the vRNAs specifically by using the MHV-specific oligo targeting the 3'UTR.
Specifically, by using an existing finite element model, the formulation provides an effective way to calculate the dispersion curves of realistic thin-walled structures.
Specifically, by using our algorithm, each agent is assigned some part of the calculation such that the agents' shares are exhaustive and disjoint.
Specifically, by using the evolutionary game theory, a spectrum-selection approach for the evolution process of secondary users is designed to converge to the evolutionary equilibrium gradually.
Specifically, by using syntactic parsing and sentiment dictionary, we propose a rule based approach to extract topic words of opinion sentences associated with negative sentiment, which are regarded as the advertising keywords.
In the MIMO radar context, it is sometimes more accurate to model the radar clutter as a non-Gaussian process, more specifically, by using the spherically invariant random process (SIRP) model.
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During the second-round PCR, the first-round PCR product could be specifically amplified by using the tag-specific primer (tag-F 5'-ATGCCACGTAAGCGAAACTC-3') and the LTR primer (LTR-R 5'-AGGCAAGCTTTATTGAGGCTTAAG-3') designed by PrimerExpress (Applied Biosystems) using default settings.
For genotyping of MLPK, the functional MLPK allele was specifically amplified by using the PCR primers wtMLPK-F and wtmMLPK-R, and the mutated mlpk allele was specifically amplified by the mMLPK-F and wtmMLPK-R primers.
Interference studies indicate that the AA could be specifically detected by using this wavelength shift-based fluorescence probe, and the QDs with larger diameter have better anti-interference performance.
All B. anthracis isolates (n = 92) were specifically detected by using the genomic TaqMan® PCR assay whereas 236 strains belonging to 19 Bacillus species other than B. anthracis were PCR negative.
GFP was specifically detected by using 1 1,000 fold diluted GFP monoclonal antibody (Covance, California).
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CEO of Professional Science Editing for Scientists @ prosciediting.com