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It was also observed that nonparenchymal cells expressing vimentin or desmin specifically adhered to GlcNAc-bearing polymer-coated dishes.
Empty wells that did not contain HCT-8 cells were used as the controls to calculate the number of bacterial cells that specifically adhered to the epithelial cells.
HT1080 human fibrosarcoma cells specifically adhered to pentameric E-cadherin, and this adhesion was inhibited by anti-functional monoclonal antibodies directed against the integrin α2 and β1 subunits, but not by a series of antibodies recognising other subunits.
For specifically adhered integrin liposomes, the addition of SN529 yielded a frequency increase of 135±13 Hz and a dissipation decrease of −26.9±1.1×10−6.
Pure integrin also specifically adhered to Fg and Fn with frequency changes comparable to the binding of integrin liposomes on both RGD-containing ECM proteins.
Inspection of the samples by light and fluorescence microscopy revealed very high numbers of EcM1 luxSAgfp and EcM1 luxSAtir bacteria (red fluorescence) specifically adhered to HeLa cells expressing GFP or TirM, respectively (green fluorescence).
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This paper describes a prospective bioadhesive liquid dosage form designed to specifically adhere to the oesophageal mucosa.
Finally the binding control, the bacterial number, which adhered to the non-specific binding sites of the well, was subtracted from the calculated data to determine the final number of specifically adhering bacteria.
Thus, to avoid confusion, we have used beads that specifically adhere to ICAM-1.
We started by specifically adhering integrin liposomes and pure integrins to Fg-coated SiO2 sensors.
Preliminary in vitro studies were also performed to evaluate the efficacy of the MIPs to specifically adhere to H. pylori.
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