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RT-PCR analysis (Fig. 8B) revealed that NKPSG cells expressed the specific transcripts for IL-21 (600 bp).
Specifically, Ago2 functions as an endoribonuclease in a protein complex bound to short RNAs that serve as guides to target specific transcripts for degradation (Reviewed in [32]).
Hence, it is important to catalogue expression of specific transcripts for as many genes as possible.
Therefore, these DEGs could be utilized as major target genes for tissue specific transcripts for tissue specific function and novel gene identification in specific tissues.
In agreement with previous findings (Topczewska et al, 2006), we found expression of specific transcripts for Nodal in 11 out of 15 (73%) analysed cell lines.
When looking at the specific transcripts for each T-cell subset we found differences in expression of Klrs, two members in CD4+ and four in CD8+.
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PCR products could be amplified in the brain transcript for exons 0a, 1b, and 2. This is identical to one of the two tissue specific transcript for adipose tissue identified by Dalla Valle et al. 2006.
As a first step towards determining any potential SXL-mediated regulation, we analyzed the tissue-specificity of the sex-specific transcripts for five of the remaining six candidates.
The amount of unique gene-specific transcripts for each specific transition compared with other transitions were not specifically examined.
The xylem-specific transcripts for most of the genes of the lignification and xylan pathways were identified and several full-length sequences obtained.
Importantly, this method makes feasible the real-world clinical implementation of recently discovered CTC-based RNA biomarkers with the potential to inform clinical decisions, such as AR-V7 in prostate cancer and liver-specific transcripts for the early detection of hepatocellular carcinoma37.
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