RT-PCR analysis (Fig. 8B) revealed that NKPSG cells expressed the specific transcripts for IL-21 (600 bp).
Specifically, Ago2 functions as an endoribonuclease in a protein complex bound to short RNAs that serve as guides to target specific transcripts for degradation (Reviewed in [32]).
Hence, it is important to catalogue expression of specific transcripts for as many genes as possible.
Therefore, these DEGs could be utilized as major target genes for tissue specific transcripts for tissue specific function and novel gene identification in specific tissues.
In agreement with previous findings (Topczewska et al, 2006), we found expression of specific transcripts for Nodal in 11 out of 15 (73%) analysed cell lines.
When looking at the specific transcripts for each T-cell subset we found differences in expression of Klrs, two members in CD4+ and four in CD8+.
Gene specific transcripts for CrWRKY49, CrWRKY50, CrWRKY51, and CrWRKY52 could not be detected in root, stem, leaf, or whole plant samples.
Throughout the paper, the number of genes identified is reported, while the Additional files contain information on the specific transcripts for each gene, including multiple transcripts identified for the same gene.
For each type, the total number of sequences, % of total expression among all venom gland specific transcripts for all sequences of this type, the proteomic evidence for the presence of the type in venom and the proposed function are shown.
This discrepancy between the numbers of sex specific transcripts for genes involved in reproduction is likely caused by the small representation of males within the Daphnia cultures used to create the cDNA libraries.
