Sentence examples for specific target sequences from inspiring English sources

It has been shown that Pbx family members, and their Drosophila melanogaster counterpart Extradenticle (Exd), function as cofactors for Hox proteins; they can enhance their binding specificity and affinity for specific target sequences in DNA [6] [10].

They can be thought of as "molecular scissors," cutting the DNA at specific target sequences.

Although the design of highly specific target sequences is essential for reducing the off-target activity, depending on the gene, it may be difficult to find the appropriate sgRNA because the GC-rich NGG PAM sequence does not frequently appear in the AT-rich Dictyostelium genome.

However, current array identification schemes are severely compromised by nonspecific hybridization, resulting in numerous false-positive and false-negative calls, they lack an adequate internal control for assessing the quality of identification, and are dependent on amplification of specific target sequences which introduce biases.

This technique utilizes reverse transcriptase (RT) to convert RNA into complementary DNA (cDNA), a thermostable DNA-dependent DNA polymerase for the amplification of specific target sequences and target specific probes (oligonucleotides) labelled with fluorophores for the detection of amplified DNA (Gibson et al. 1996).

Further, it will be important to identify interacting partner proteins of Mesp1 and Mesp2, which could be able to form a protein complex together with Mesp1 or Mesp2, functioning in strict post-translational control or modulating the transcriptional activities by determining specific target sequences.

Kikuchi et al [ 2] identified specific target sequence of PAX3-FKHR (GCCTCTCACCTCAGAATTC) and designed corresponding siRNA (GCCUCUCACCUCAGAAUUC) to specifically target PAX3-FKHR without affecting either PAX3 or FKHR.

To avoid potential risk of off-target effects, each sgRNA was designed to recognize a specific target sequence that does not share homology with any sequence of mouse genome.

The engineered DNA binding protein or RNA sequence guides the nuclease to a specific target sequence in the genome to induce a double strand break.

The probe DNA containing BDF nucleosides exhibits a drastic change in fluorescence intensity only when it hybridizes with a specific target sequence.

In the conventional MLPA approach, one of the two target probes is generated by cloning in a single-stranded bacteriophage vector to introduce a sequence of defined length between the primer binding site and the specific target sequence.