Exact(30)
barcoded 3′-end specific sequencing.
A further challenge is posed by the specific sequencing technology employed.
A specific sequencing primer was designed for H5N1, with no sequence similarity to the other influenza virus HA subtypes based on the database searches and alignments (Figure 1).
This approach has the same resolution as allele specific sequencing.
This technique has the same resolution as allele specific sequencing.
The ISSH method is not linked to a specific sequencing technology.
Similar(30)
The simulated sequences contained Illumina-specific sequencing errors (see Methods).
Sample-specific sequencing quality scores (percentage of bases with quality ≥ Q30 and average Q-score).
SOLiD™ sequencing-specific sequencing adaptors are then ligated to the ends of these fragments.
Besides, the noises in the data (both platform-specific sequencing error and artificial chimeric reads) impede the specificity of SV detection.
In response, NEB has now developed a kit called NEBNext Ultra to produce strand-specific sequencing libraries.
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