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For this purpose, we reverse transcribed RNA isolated from the above tissues and PCR-amplified specific sequences of the Lef1 and Tcf7l2 cDNA with primers designed to span specifically the alternative splicing regions.
Two PCR primers and a hybridization probe were designed to target specific sequences of the S. intermedius thermonuclease (nuc) gene.
To detect and distinguish these species, a loop-mediated isothermal amplification (LAMP) platform that targets specific sequences of the internal transcribed spacer (ITS) genes was developed.
It remains to be determined what specific sequences of the HcpA protein contribute to the eukaryotic proinflammatory activity demonstrated here.
The qPCR targets Leptospira spp. specific sequences of the 16S rRNA gene present in two copies in the genome [1], [8].
DNA was extracted from bacterial pellets of B. cenocepacia (strain J2315) and P. aeruginosa (strain PAK) with the DNA tissue culture extraction kit (Qiagen) Forward and Reverse primers were designed based on TcPRAC sequence toward specific sequences of the genes of interest (Table S2, Supplemental files).
Similar(42)
The method depends on adaptors that bind to a specific sequence of the four DNA letters on the tail of each bead's gene.
Beside these original patterns related to the specific sequence of the peptides, the next step and the main objective is to control the attachment of nanoparticles on carbon nanotubes.
The design of molecules that recognize the specific sequence of the DNA double helix or those that can stabilize DNA topoisomerase cleavable complex to stop the progression of DNA process, may be very useful in cancer chemotherapy.
In 1999, DNA was extracted from leg muscles of 100 wild caught cowbirds and subjected to a PCR targeting an S. neurona specific sequence of the small subunit ribosomal RNA (SSU rRNA) gene.
This suggested that the specific sequence of the ITR5 spacer plays an active role in the Rep5-ITR5 interaction.
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