Sentence examples for specific sequences from the from inspiring English sources

The researchers propagated individual DNA fragments in the traditional way, in bacteria, to create a gene library; they also developed a method for pulling out specific sequences from the library, providing an easy way to study any gene of interest.

H1299 cells in 6 cm dishes were grown to 80% confluence and transfected with 0.5 µg of a plasmid containing the luciferase reporter gene fused to p53 specific sequences from the p21 promoter (p21-Luc) [41], 0.01 µg pCDNA3.1-p53 pCDNA3.1-p5353 [40] (both plasmids kindly providexpressingam Benchimol) and 4 µg of a either p53smid expressing the USP7-CTD or empty plasmid.

The second pair contained 56-bp and 60-bp specific sequences from the 5' and 3' ends of human HPRT.

After selecting the specific sequences from the target protein, the siRNAs may be synthesized using commercial kits or via a commercial laboratory.

A pair of universal primers for prokaryotes (total number of microorganisms) was used, and pairs of primers that allow amplification of specific sequences from the chromosomal DNA of bacteria of the genera Lactobacillus and Bifidobacterium.

Sequence of all inserts was confirmed from both the plasmid and the virus supernatant (we were able to obtain high-quality specific sequences from the virus supernatant using universal primers, most likely due to trace amounts of plasmid).

To ascertain the degree of bias in a given sample, a relative value for each specific sequence from the observation region was calculated.

To obtain laccase-gene-specific sequences from the white-rot fungus Trametes sanguinea M85-2, a PCR screening method was used.

Homoeolog-specific sequences from the tetraploid species were assigned to the A or B genomes by BLAST to the bread wheat chromosome arm-specific genomic survey data above (Table  1).

Four different strains – ΔserB, ΔgltA, ΔilvA, and Δfes – were rescued by specific sequences from our library.

Potential novel miRNAs were discarded from the final collection when they were reported as deriving from heterogeneous precursor positions or there was no clear dominance of their specific sequence from one arm of the proposed hairpin structure.