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For this purpose, fifteen high risk HPV type specific reverse primers were designed on L1 gene and fluorescently labeled.
With respect to specific reverse supply chains factors, the only significant one identified is the percentage of units returned.
e Screening of putative transgenic lines for the presence of OsMKK6 transgene by total genomic PCR using pCAMBIA specific forward and OsMKK6 specific reverse primer.
The analysis of non-defined environmental samples may require a species specific reverse primer to ensure only Methanospirillum hungatei JF1 is detected.
DinoSL was used as the forward primer paired with the gene specific reverse primers (Table 1).
The PCR reactions for pyrosequencing were done with a biotynilated specific reverse primer and performed as follows.
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The kit includes the Multiscribe™ Reverse Transcriptase and a specific reverse-transcription primer against each miRNA.
In the B224-specific qPCR, the specificity is established by the B224-specific reverse primer that only anneals to Symbiodinium B224.
First-strand cDNA synthesis was obtained using ML1654 as gene-specific reverse primer.
For miRNA expression analysis, cDNA was synthesized with stem-looped miRNA-specific reverse transcription primers by using the same kit.
We additionally confirmed the SC-specific expression of H3mm7 using variant-specific reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) Supplementary Fig. 2a), as used for H3.3-coding genes (H3f3a, H3f3b) (Supplementary Fig. 2b).
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