Since experiments were independent of each other, specific replicates of a given strain were not directly linked between all of the experiments.
The CMH is sensitive to the specific replicates included, as including the fifth replicate in the optimal sequence actually leads to worse performance than four replicates (Supplementary Fig. S3c and d).
In our dataset the median CV was 0.30 among replicates (min = 0.01, max = 2.59), calculated over all probes in all prominence- and time-specific replicate groups (Figure 3).
A normalized intersection network was calculated by comparing the two species-specific replicate intersection networks.
Controls for experimental variance were performed by constructing two replicate-specific networks for human and mouse respectively and then computing the species-specific replicate intersection networks.
We start with an ANOVA model for the log transformed miRNA expression y gcr for miRNA g, experimental condition c (c = 1, 2), and condition-specific replicate c r, as (1) y g 1 r ∼ N a g − 1 2 δ g + β g 1 r, σ 2 g y g 2 r ∼ N a g + 1 2 δ g + β g 2 r, σ 2 g suggested by Kerr et al [ 19].
Tyrex2 was then used to regulate adenoviral E1A expression to construct a melanoma-specific replicating adenovirus.
The MLP may be a useful promoter in the context of a tissue-specific replicating adenovirus.
In this manuscript we present the first description of an episomal and tissue-specific replicating SMAR-based vector.
