Unlike GalK insertion, which has an efficiency close to 100% in MVA-BAC, the specific replacement of GalK with an exogenous sequence has been achieved to date at a frequency of only up to 1% compared to spontaneous deletions.
In addition, we investigated if a reduction of Polo dosage in bubR1 mutant females allows a higher efficiency in the specific replacement of cohesin complex by condensin complex during chiasmata formation (Yu and Koshland 2005) and SC disassembly (Ivanovska et al. 2005; Resnick et al. 2009).
This specific replacement of surface amino acids with either acidic or basic amino acids was termed supercharging, as the net charge of the mutants were more positive or negative [ 8].
The energetic consequences of site-specific replacement of a residue at a partially buried site in the two homologous HPr proteins from Escherichia coli and Bacillus subtilis is described.
More recent studies have confirmed the functional importance of increased cTnI phosphorylation in endotoxemia by demonstrating that mice with cardiac-specific replacement of cTnI with slow skeletal TnI (ssTnI), which lacks the PKA phosphorylation sites in the N-terminal, are significantly protected against LPS-induced cardiac dysfunction.
Thus, species-specific replacement of either of these two residues can act as a conformational switch, impacting the overall structure of PrP23-144 amyloid.
Thus, species-specific replacement of either of these residues could act as a "conformational switch" that ultimately impacts the overall structure of PrP23-144 amyloid.
MCY Chang, CS Yee, DG Nocera, and J Stubbe (2004) Site-specific replacement of a conserved tyrosine in ribonucleotide reductase with an aniline amino acid: A mechanistic probe for redox-active tyrosines.
The increasing selenium toxicity with sulfur deficiency indicates interference of Se with sulfur metabolism, possibly resulting from non-specific replacement of sulfur by selenium in proteins and other sulfur compounds.
Specific heterocycle replacements of the phenyl rings in AGSMs provided potent molecules with improved aqueous solubilities.
