Exact(9)
Minor grove binder (MGB) modifications at the 3'-end of sequence specific oligos provide the advantage of designing short probes with higher melting temperature (Tm) and increased duplex stability and specificity in comparison to conventional Taqman probes [32], allowing for multiplexing and specific mismatch identification as in the case of single nucleotide polymorphisms (SNPs) [33].
The measurements in the temperature range of 25 600°C document a change of stresses in GaN and AlN layers, as interpreted by the specific mismatch of thermal expansion coefficients.
Surveys of a range of bacterial genomes have found little correlation between the presence of specific mismatch repair genes and the equilibrium GC content of the genome [ 27].
As specific positions of mismatches affect miRNA targeting [ 37, 38], we also collected these positions to enable the query of predicted targets with specific mismatch positions of interest.
The dCAPS marker was designed with a specific mismatch in the primer to introduce a restriction enzyme Hpy166II recognition site using an available program dCAPS Finder 2.0 (http://helix.wustl.edu/dcaps/dcaps.html).html
Note that the PM/MM-gain α ≈ log(s) approximately refers to the free energy increment of one Watson-Crick pairing in 25 meric probe/target duplexes if one neglects the specific mismatch contribution.
Similar(51)
This paper also estimates the following standard wage regressions for each of the participating countries to assess the country-specific mismatch wage penalties.
Species-specific mismatch repair genes in different plant taxa may likewise play a role in the widespread lack of consistent patterns across taxonomic clades.
Gene-specific mismatch oligos including trpm7 5-mispair-MO (5′-CCtrpm7 5-mispair-MOtrpm7 5-mispair-MO3a-5′-CCtGcACTTgTGGcACATTgTCTTC-3′cCGandgACAC-3′) (lowercase indicates mismatched bases), as well as standard control oligosocs3a-5-mispair-MOTTACAATTTATA-3′) that have been shown not to be directed against any zebrafish gene sequence, were used as controls.
Under our model the probability of a sequencer generating m p mismatches in l reads at read position p is: Where l is the number of reads, m p is the observed number of mismatches in all l reads at read position p and is the position-specific mismatch rate.
Patient-specific mismatch-related quantification errors (MRQE) were calculated as the differences between the log10-transformed output copy numbers of the individual mismatched templates and the log10-transformed median output copy number of the templates without mismatches.
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