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Sequencing data were segregated by the individual specific MID.
Sequencing data for each individual were then extracted according to the specific MID.
The ends were polished and the 454 titananium adapters containing specific MID sequences were attached.
Confidence in a specific MID value evolves over time and is confirmed by additional research evidence, including clinical trial experience.
In total, two independent pyrosequencing runs were performed (14 samples with specific MID were pooled in each).
To make multiplexing possible in both cases of sequence capture and sequencing, each library was prepared with a specific MID (Multiplexing Identifier) on its 454 adapters (Lib-L).
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Throughout 2009, in a series of interviews, Kamal laid out his evidence, using maps that plotted the routes used by jihadists to cross the border into western Iraq, and confessions that linked their journeys to specific mid-ranking officers in Syrian military intelligence.
If you intend to look for a faculty job after an industry postdoc, then you essentially need to take a very hard look at specific companies that either have a reputation for strong research divisions (where the publication rates are relatively high for industry) or at specific mid-size start-ups that rely on strong research.
During library preparation, sample-specific MID adaptors (Table 1) were blunt-end ligated onto each DNA fragment in a sample.
However, "non-specific mid back pain", although less common may have a similar (as yet largely unknown) etiology and course as non-specific low back or non-specific neck pain.
Two different primer pairs were designed for PCR 1 (locus specific amplification) and PCR 2 (OTU-specific MID attachment) (Fig. 1 A and B ). From 5′ to 3′, the primer pair for PCR 1 was composed of an adapter of 21 bp (GAA GGT GAC CAA GTT CAT GCT) provided by Fluidigm Inc. and a locus specific forward and reverse primer, usually 20 25 bp in length (see Targeted Genes).
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