Each specific maker with appropriate compound SSR primers was examined the male specificity.
Primary antibodies were goat polyclonal anti-Brn3a antibody (1 500; Santa Cruz Biotechnology, Santa Cruz, CA, USA), a specific maker for RGCs [ 30], monoclonal mouse anti-GFAP antibody (1 500; Sigma, St . Louis MO, USA) and rabbit polyclonal anti-Iba1 antibody (1 500; Wako Chemicals USA, Inc., Richmond, VA, USA).
26 We therefore examined whether cells at the periphery of the SH-IN 4F colonies had lost expression of specific markers of pluripotency (TRA-1-81 and OCT4) and recovered expression of the neuronal specific maker (βIII tubulin; Suppl. Fig. S9).
The retinas were blocked in PBS containing 3%% donkey serum, 1 % bovine serum albumin, 1 % fish gel and 0.1 % Triton X-100 for 1 h, and incubated with goat polyclonal anti-Brn3a antibody (1 500; Santa Cruz Biotechnology, Santa Cruz, CA, USA), a specific maker for RGCs [ 28, 29], and guinea pig polyclonal anti-GFAP antibody (1 500; Advanced ImmunoChemical, Long Beach, CA, USA) for 3 days at 4 °C.
(K) Immunofluorescence analysis shows NSCs generated from iPSCs express specific makers SOX2, Musashi, Nestin, and PAX6; and neurons generated from iPSCs express neuronal makers MAP2 and TUJ1.
CEA, epithelial membrane antigen (EMA), pancreatin, chromogranin A, CD56, and synaptophysin are specific markers to identify carcinomatous components, whereas desmin, vimentin, and α-smooth muscle/sarcometic actin are specific makers to identify sarcomatous components [9].
Meanwhile, expression of neural specific makers, e.g., PAX6 and SOX1, increased during differentiation (Figures 1C).
In the present study, we report for the first time that hESC extract induces reprogramming in human fetal fibroblasts (HFFs) as determined by morphological changes and re-activation of ESC specific makers.
Adipogenic progenitor cells were sorted from the adipogenic progenitor specific maker-labeled SVF suspensions with AriaII (Becton Dickson) cell sorter, and the purity of sorted cells verified as >95% by rerunning the sorted population.
For all ex vivo analysis, PDZ-RhoGEF expression, progenitor cell proliferation, and PI3K signaling, adipogenic progenitor cells were sorted from adipogenic progenitor specific maker-labeled SVF suspensions with AriaII (Becton Dickson) cell sorter, and the purity of sorted cells was verified as >95% by rerunning the sorted population.
Our clone of differentiated immortalized cells could propagate in standard culture condition for greater than 6 months with sustainable hepatocyte specific makers and functions.
