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The glycosyltransferases utilize unique cosubstrates, which are generally a sugar donor along with an acceptor molecule with specific linkage specificity.
In order to facilitate the specific linkage of the DOTA moiety in the peptide dimers, the N-terminal amino acid of the sequence was introduced as an Fmoc derivative except when the DOTA moiety was coupled to the N-terminus.
A total of 27 newly-designed microsatellite markers were added to a previously generated map and then localized to specific linkage groups, using a de novo single-pair cross, in order to demonstrate the utility of the tool.
Also, the geometry of cyanide bridges and the preference of metal ions for a specific linkage isomer allow predicting oligonuclear structures based on relatively simple building blocks of low nuclearity.
Two pairs of linkers: biotin-streptavidin and digoxigenin-anti-digoxigenin, are chosen to permit specific linkage of the DNA to a polystrene microsphere and glass coverslip, respectively.
How does a specific DUB recognise a specific linkage type?
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The genomic inflation factors from locus-specific linkage analysis were 1.69 (SE = 0.21, p = 0.04) for BMI and 2.18 (SE = 0.21, p = 2 × 10−10) for height.
Moreover, exploiting the different binding energies of chlorine, bromine, and iodine toward the phenyl group has enabled sequential and site-specific linkage as a promising approach toward hierarchical design.
To date, however, no genome-scale parent-of-origin-specific linkage analysis has been reported for ASD.
The linkage distances for sex-average and sex-specific linkage groups was estimated assuming the Kosambi mapping function [54].
Enrichment was also found for cytobands representing chromosomal regions not previously described in the disease that could potentially guide future region-specific linkage mapping in AE.
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