Sentence examples for specific inserts from inspiring English sources
The cDNA is inserted directly into the M13 vector, which is screened by plaque lifting for the presence of specific inserts.
The presence of the respective construct in the transformants obtained was confirmed by PCR on DNA extracted from the different colonies using specific oligonucleotides for the specific inserts.
"This is a movie that often seems to be missing important transitional scenes or specific inserts," wrote Vulture's Kyle Buchanan in a hilarious must-read review of the film, "but you had better believe that when Nicole Kidman pees on Zac Efron, that camera is there".
This is not surprising considering that most genetic selections require multiple rounds of re-selection to enrich for specific inserts [12].
Specific inserts were sequenced using T7 and M13 forward sequencing primers.
Membranes were probed with P-labelled probes made from gene specific inserts represented in the microarray analytical results.
The recombinant clones having the specific insert in the forward orientation was then sequenced from both directions to get a contiguous sequence in an automated sequencer (ABI prism, model 377, Version 3.0).
In addition, we provide evidence that the tumor-promoting activity of IRP2 requires its specific insert of 73 amino acids.
Human H1299 lung cancer cells or clones engineered for tetracycline-inducible expression of wild type IRP2, or the deletion mutant IRP2Δ73 (lacking a specific insert of 73 amino acids), were injected subcutaneously into nude mice.
Subsequently, the variant 1 or 2 specific insert sequences of 9 randomly picked clones were determined by PCR using an exon 2 located reverse primer and an exon 1a-specific forward primer, which allows the amplification of both variants with one primer set, leading to size-specific variant 1 (87 bp) or variant 2 (98 bp) products (Figure 7B).
We examined split 8-mers in human promoters and identified pairs of 4-mers that localized at a specific insert length and not others.
