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Fragmentation was carried out on all UDP-sugar standards seeking for specific fragments that would indicate the certain UDP-sugar.
The MS MS fragmentation conditions were optimised for each compound in order to obtain both specific fragments and high signal intensity.
Chen, L. et al. Identification of specific fragments of HpaGXooc, a harpin protein from Xanthomonas oryzae pv.
Two specific fragments were found and their DNA sequences were utilized for primer design.
The answer came when it was found that specific fragments of cell wall polysaccharides, called oligosaccharins, are able to induce specific responses in plant cells and tissues.
The primers designed generated specific fragments of 84, 121 and 122 pb length for bovine, ovine and caprine species, respectively.
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The size of the SCAR fragments was concordant with that of the RAPD species-specific fragments.
One hundred primers (Operon kits OPAA, OPAO, OPAV, OPC, and OPE series) were used for random amplified polymorphic DNA (RAPD) fingerprinting to determine male-specific fragments.
From the nucleotide sequence, PCR primers were designed to amplify 553-, 895-, and 194-bp sex-specific fragments present in all female S. cheela hoya.
In addition, 120 random primers were used for RAPD fingerprinting to search for novel sex-specific fragments of S. cheela hoya.
All primer pairs produced virus-specific fragments in both simplex and multiplex formats, thus confirming the efficacy of the newly developed mRT-PCR for detection of these viruses.
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