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This method utilizes unlabeled monoclonal antibody (mAb) P2G10 and biotin-labeled, mAb P2C11 (BD/Pharmingen, San Diego, CA) that are specific for different epitopes of porcine IFN-γ [13].
Here the term memory cell is not used in the strict meaning of one single cell but as an abstract unit of memory responses, such as different cells specific for different epitopes of the same pathogen or different types of memory cells.
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Different Tg assays employ a number of anti-Tg antibodies with varying specificity for different epitopes.
The specificity of the two antibodies for different epitopes allowed the elimination of non specific bands and showed a prominent signal at the correct molecular weight.
Each tissue was immunostained for different epitopes (CD3, CD4, and CD8) using specific monoclonal antibodies, so that comparisons could be made between the distributions of different cells within an individual tumor specimen through use of video image analysis.
Notably, antigen-presenting cells (APC) bearing peptide antigen in the context of Q115E HLA-A2 were able to induce substantially better proliferation of tumour-specific CTL clones specific for several different epitopes (Fig. 2C, D and data not shown).
It is therefore possible that different levels of inhibition acting on CTL specific for different LCMV epitopes could influence CTL killing rates and that our estimates are not the 'true' killing rates of these populations.
Biotinylated detection antibody specific for a different epitope on the cytokine was added to the beads and incubated at room temperature for 30 min.
Then, a biotinylated detection antibody specific for a different epitope on the cytokine was added to the beads and incubated at room temperature for 30 minutes.
Alternatively, we cannot exclude that T cells specific for different AQP-4 epitopes differ in their tropism to CNS, muscle, and kidney, or, that T cells with specificities unrelated with AQP4 prepare the field for NMO-IgG induced CNS pathology in NMO patients.
Some papers, there has been a relatively large variation in sensitivity and specificity figures of p-Tau between studies using ELISA methods specific for different phosphorylated tau epitopes.
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