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An interesting observation has been the stage specific essentiality of the gene.
These results are strongly indicative of the stage specific essentiality of this gene in M. tuberculosis.
But it seems logical that PPK due to its facile reversibility and its stage specific essentiality would turn out to be a prime candidate.
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Erythroid lineage-specific essentiality scores are available in Additional file 3 (Tables S4-S6).
The same framework and techniques with small modifications can however be utilized, to probe condition-specific essentiality, when sufficient data becomes available.
Using the weighted linear equation generated by the best solution (GA run 16 4; Additional file 2: Table S3) a lineage-specific essentiality score (S) was calculated for each TF.
Reasons for these genes being identified as essential in other species could be due to multiple variables such as in vitro selection media, species-specific essentiality, transposon type, library complexity, sequencing method, and criteria for essentiality.
However, the architecture of an interaction network also provides insight into specific gene essentiality in the modeled system.
Major hindrances of a cross-organism comparison are differences in experimental approaches and the specific definition of essentiality used.
Therefore, whereas panthothenate is commonly thought to be essential, this essentiality is strain specific and absent in C. difficile strain 630.
Recent efforts at manual re-annotation [ 100] and condition-specific high-throughput essentiality studies [ 38, 101] have continued to improve the existing model.
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