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After removal of the blocking solution, the membrane was incubated with a fresh blocking solution containing the primary antibody at a specific dilution (1∶10000 to 1∶25000) for 1 hour.
The culture was changed to a continuous mode at 24 h with a specific dilution rate.
Fifteen bioreactor cultures were performed at various specific dilution rates (D) ranging from 0.05 to 0.64 h-1.
For the measurement of maximal inhibition, a specific dilution below the critical actin concentration had to be applied to completely depolymerize the F-actin.
For the measurement of maximal inhibition, a specific dilution below the critical actin concentration had to be applied to completely depolymerize the filamentous (F) actin.
Inhibition percentage was calculated as the following: (RLA in virus challenge controls – RLA in test well for each serum at specific dilution)/RLA in virus challenge controls.
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Seven primary antibodies (all from Abcam, Cambridge, UK) were used at specific dilutions: 1/750 for TIMP1, 1/1000 for APOD, SDHA, CLGN, CRABP1 and APOE, and 1/5000 for α-tubulin as a control.
In addition to regularly training in calculations, written procedures for specific dilutions and infusions used in the wards would be of importance as a quality insurance for improved patient safety.
At these specific dilutions, an increase in the performance level correlated with an increase in input strength; either by an increase in the number of activated glomeruli or by an increase in the amplitude of glomerular responses.
Using the appropriate chip-specific dilution buffers, we diluted the serum samples 1 10 for use in the synovial antigen 1 and 2, CID 3, and CID 4 chips, and 1 100 for use in the CID 1 chips.
Here, defined amounts of the test item are directly weighted to the test-specific dilution water, stirred for about 24 72 h, and eluates are obtained after the separation of solids by sedimentation, filtration, or centrifugation.
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