The third explanation is the confluence of specific events and public officials.
To avoid any non-specific staining due to confluence, SA-β-Gal cytochemical staining was carried out on non-confluent cells.
There was no such finding in the Regular vs. Irregular sequence contrasts, nor was there any apparent confluence of submodality specific regularity sensitive areas.
For EGF stimulation experiments, cells were grown in cell line-specific media to approximately 80% confluence.
A549 cells were treated with either control (scrambled) or PAX8 specific siRNA, allowed to grow to confluence in 24 well plates with cell culture inserts, and then inserts were removed to create a fine scratch.
Moreover, ASM cells at confluence retain smooth muscle-specific actin, SM22, and calponin protein expression, and mobilize intracellular Ca2+ in response to acetylcholine, a physiologically relevant contractile agonist[36].
Moreover, B/TSM cells at confluence retain smooth muscle-specific actin, SM22, and calponin protein expression and mobilize intracellular Ca2+ in response to acetylcholine, a physiologically relevant contractile agonist [19].
MDCKαβ cells were grown to confluence, immunostained with a meprinβ-specific antibody, and analyzed by confocal microscopy.
Cells were initially seeded at a density of 1 × 10 cells/cm in normal medium, then converted into specific differentiation medium when they approached 80-90% confluence.
Ms. Fuller also declined to go into specifics, but said that it was "obviously a confluence of certain things that made this happen".
