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The content also adds a "challenge mode" similar to that in Portal players try to complete specific chambers with the shortest time or fewest number of portals used, both which are tracked on overall and friends leaderboards.
To address whether premature differentiation of Islet-1 progenitors might lead to a depletion of markers for the SHF and its derivatives, day 14 EBs (7+ days after the onset of beating) were analyzed for the expression of cardiac markers that become regionalized to specific chambers during development.
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Note that continuous measurement in the chamber is possible with a specialized pH microelectrode, although this may depend on the specific chamber design involved.
For the chamber geometry employed, Filgueria et al. (2006) found that a maximum of 20% depletion value was appropriate and apparently well justified as a guideline for future use of this specific chamber design.
The widely used 'flow-through chamber method' (FTC) for clearance rate measurements in mussels was validated by Filgueira et al. (2006) using a specific chamber geometry and by making a clearance rate versus flow rate plot as per Riisgård (1977, 2001a).
The maintenance of feces in the nest is a poorly studied phenomenon in eusocial insects [ 36, 37], in which colony members are generally assumed to evacuate feces into specific nest chambers to prevent microbial development in the colony (reviewed in [ 2, 38]).
Given facility limitations and the requirement of this specific exposure chamber setup to maintain coral health, the experimental design included single exposure chambers containing five independent biological replicates.
A specific isolated chamber is used as a latrine, while another is used as a nest.
The membrane was put in a specific filtration chamber previously designed [1], allowing direct on-line microscopic observations of particles deposition.
To this purpose we have designed a specific flow chamber and we have tested it with osteoblasts grown on an opaque, thick support, made of smooth or sandblasted titanium.
That's why today's #WomenBoycottTwitter action feels off to me and why I choose not to participate in something that is designed to rally "us" but appears like it was designed in a very specific echo chamber that represents a narrow and privileged subset.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com