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All primers (Additional file 5: Table S1) were optimized using SYBR Green (Applied Biosystems, Life Technologies, Grand Island, NY, USA) and melt curve analysis to ensure that amplicons were specific and free of primer-dimer products.
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To give consumers a reason to follow along with your conversation offer regular coupons, industry specific tips, and free resources.
These programs included specific training and free nicotine replacement treatment for pregnant smoking women throughout pregnancy [ 24].
We used several assays for detecting cellular and tissue ROS, as none of them is specific and artefact free.
The observer did not participate in the family interactions during the diabetes-specific situation and free play situation.
The contents are fairly basic but it is accurate, helpful on the specific topics addressed, and free.
But, then again, it is also worryingly short on specifics and suspiciously free of concrete commitments.
In women with colorectal carcinoma, total prostate-specific antigen positivity was 20% and free prostate-specific antigen positivity was 34.6%.
Animals were housed in specific pathogen free and light, temperature (21°C) and humidity (50 60% relative humidity) controlled conditions.
The presence of nodal metastases had a significant negative association with disease-specific and disease-free survival [78].
Overall, cancer-specific, and recurrence-free survivals were assessed.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com