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Based on the results of the species validation method, we propose that at least two species of Prasiola associate with the lichen-forming fungus Mastodia tessellata.
The mtDNA CR was considered ideal for species validation because previous population-based studies based on this genetic marker had already analyzed hundreds of tuna individuals and had detected mtDNA introgression among T. thynnus, T. orientalis and T. alalunga species [15].
Species validation of the hormone assay was performed previously by Franceschini et al. [14].
This range of species should provide an approximate starting point for new researchers utilizing this technique; however, any researcher replicating this protocol should perform a species validation.
Whole cell protein (WCP) fingerprinting of enterococci were done for species validation by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and analyzed computationally.
The requirement of other platforms for either surface immobilization and/or labeling steps makes assay development and species validation arduous, slow, and expensive.
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Moreover, genetic distance estimates and haplotypic differences were useful for species validations.
Thus, to our knowledge, this is the first cross-species validation of [TIMP-2]·[IGFBP7] for AKI and establishes the test as a viable tool in the preclinical space.
The processing of all 8 species took ∼3,000,000 CPU hours including cross-species validation.
We can significantly improve the precision of weak conserved domain predictions by cross-species validation of HMMerThread hits.
Such studies would be valuable for not only the cross-species validation of the Sp4 mouse model but also the development of gene-specific diagnosis and treatment.
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